CD14 antibody (Biotin)

Catalog No. ABIN111908

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD14 in samples from Human.

Quick Overview for CD14 antibody (Biotin) (ABIN111908)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-8

Product Details anti-CD14 Antibody (Biotin)

Purification: Affinity chromatography.

Isotype: IgG2b

Application Details

Application Notes: Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 μg/mL,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15 % positiveHL-60 stimulated with DMSO 15 % positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 5 mg/mL bovine serum albumin (BSA) as a stabilizer without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. This product should be stored undiluted.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 9606

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades